wyguo / ThreeDRNAseq

A pipeline for differential expression and differential alternative splicing analysis
https://github.com/wyguo/ThreeDRNAseq
GNU General Public License v3.0
61 stars 26 forks source link

[Help] error with time series analysis #42

Open Shukla04 opened 1 year ago

Shukla04 commented 1 year ago

Thanks for using our 3D RNA-seq App.

Describe the question Please ask questions about how to use the 3D RNA-seq App or to understand why the App is not working. I want to do time series trend analysis. I have samples over an 24 hour cycle from 4 time points(T1-T4):- 30min, 2hr, 7hr and 10hr after treatment. I don´t know how to generate groups with them as they are not from different days. One group has only one time point in this case. I want to see the trends of gene expression throughout this 24hr cycle. Please help me and guide me on how can I proceed.

I had tried to generate 4 groups with each time point and 4 different time points in total (each time point has two biological replicates). Every time I run the shiny App, it get stuck and I do not get any trend or result. Eventually, i had to terminate the App.

Screenshots If applicable, add screenshots to help explain your questions. 3DRNAseq_time series