I have a question regarding the default implementation of MCScanX,
As the manual suggested, I first run blastp and obtained the .gff and .blast file that were used as input for MCScan. In the .blastp file, I have noticed gene pairs with alignment e-value as low as 1e-110. However, these matched gene pairs were not shown in the .colinearity file after I run MCScanX. I have read through the manual and the paper, but still couldn't find any explanation for that. Can anyone please clarify?
Hello,
I have a question regarding the default implementation of MCScanX, As the manual suggested, I first run blastp and obtained the .gff and .blast file that were used as input for MCScan. In the .blastp file, I have noticed gene pairs with alignment e-value as low as 1e-110. However, these matched gene pairs were not shown in the .colinearity file after I run MCScanX. I have read through the manual and the paper, but still couldn't find any explanation for that. Can anyone please clarify?
Thanks in advance, Mandy,