Hi, there,
I found missing homologs in my results, though they show 100% homolog in my blastp results.
I tried different parameters, checked all my command, nothing wrong, and I found this issue occurred more than 5 times in my overall results.
Please help me solve this:
Hi, there, I found missing homologs in my results, though they show 100% homolog in my blastp results. I tried different parameters, checked all my command, nothing wrong, and I found this issue occurred more than 5 times in my overall results. Please help me solve this:
` 1 V.g1576 tig00000012_np1212.g1452
1 V.g1577 tig00000012_np1212.g1453
1 V.g1578 tig00000012_np1212.g1454
1 V.g1579 | |
1 V.g1580 | |
1 V.g1581 tig00000012_np1212.g1457
1 V.g1582 tig00000012_np1212.g1458
1 V.g1583 tig00000012_np1212.g1459
I used these commands:
blastp -query JYF80.pp.fa -db Sc2.pp -out 80_Sc2.blast -evalue 1e-5 -num_threads 16 -outfmt 6 -num_alignments 5 MCScanX 80_Sc2 -e 1e-5 -s 10