I am new to metcat and attempting to assemble amplicon based sequence reads for a virus genome. I am wondering on the set of optimal parameters for a 16000 long genome with 1500-2500 long amplicon sequences. In particular, how do I set the error rate parameter? why cant metcat deduce this from the data?
I am new to metcat and attempting to assemble amplicon based sequence reads for a virus genome. I am wondering on the set of optimal parameters for a 16000 long genome with 1500-2500 long amplicon sequences. In particular, how do I set the error rate parameter? why cant metcat deduce this from the data?