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xie186 / ViewBS

ViewBS - a powerful toolkit for visualization of high-throughput bisulfite sequencing data
GNU General Public License v3.0
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Closed shenweima closed 5 years ago

shenweima commented 5 years ago

In fact, an error was found using test data according to command of ViewBS MethOneRegion --region chr2:2000-10000 --sample test_WT.tab.gz,test --prefix chr2:2000-10000 --context CHG

Output prefix: chr2:2000-10000
Start calculate methylation information for target context
CHG
Can't call method "next" on an undefined value at /root/software/ViewBS/lib/Meth/OneRegion.pm line 60.

I have downloaded docker, but this error was also found

image I downloaded the test file again and now it works well

gourav924 commented 2 years ago

Did you figure out what was the reason for the error? I am stuck at it as well. I am using it on my machine

Thanks!

yykaya commented 1 year ago

Hi gourav94, This error is due to whether the total number of scaffolds in the reference genome is consistent or not. I recommend you to index and try the assembled genome in your project.