xinehc
commented
1 year ago Hi,
this could be caused by missing certain binaries or incompatibility in Windows, will try to fix but it may take some time, please use the command line version for customised nucleotide database ATM.
fengyuan1112
首先，十分感谢贵课题组的ARGs-OAP给我们这种生信刚入门的小白提供了研究ARG的便利工具，我最近在尝试使用Desktop版本（windows）进行个性化数据库分析，已经成功使用Ncyc等多种数据库进行分析，但是当使用同样的方法进行Nature文章中所提供的MGEs数据库进行分析时，总是会出现如下错误，恳请您们帮忙指点迷津，十分感谢 [2023-07-25 00:13:06,443] [INFO] Calling args_oap/stage_one with ['stage_one', '--indir', 'D:\Metagenomics\ARGs-OAP\input\test_P', '--outdir', 'D:\Metagenomics\ARGs-OAP\input\test', '--thread', '2', '--format', 'fa', '--skip', '--database', 'D:\Metagenomics\ARGs-OAP\MGEs\MobileGeneticElementDatabase-master\MGEs_FINAL_99perc_trim.fasta'] [2023-07-25 00:13:06,457] [INFO] Building databases ... [2023-07-25 00:13:20,312] [INFO] Processing <D:\Metagenomics\ARGs-OAP\input\test_P\E1_final.contigs.fa> (1/1) ... [2023-07-25 00:15:35,473] [ERROR] Traceback (most recent call last): File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\interface.py", line 474, in start_new_job File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\modules\args_oap\api.py", line 77, in call_stage_one File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\modules\args_oap\stage_one.py", line 210, in run_stage_one File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\modules\args_oap\stage_one.py", line 190, in run File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\modules\args_oap\stage_one.py", line 172, in extract_seqs File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\subprocess.py", line 548, in run File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\subprocess.py", line 1024, in init File "C:\Users\ADMINI~1\AppData\Local\Temp\ONEFIL~1\subprocess.py", line 1509, in _execute_child FileNotFoundError: [WinError 2] 系统找不到指定的文件。
[2023-07-25 00:15:35,473] [ERROR] FileNotFoundError(2, '系统找不到指定的文件。', None, 2, None), please check whether you have installed necessary modules.