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xryanglab / RiboCode

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MIT License
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No obviously periodicity are detected from bam file when running metaplots step #62

Open DooYal opened 8 months ago

DooYal commented 8 months ago

When I process metaplots, the log printed that:

No obviously periodicity are detected from bam file SRR22462570/SRR22462570.Aligned.toTranscriptome.out.bam, it could be due to poor quality sequencing. Please check the metagene plots and try again by lowering the value of frame0_percent

Even thought used the following parameters metaplots -a $genecode.v44.ribo.anno -r $sample.Aligned.toTranscriptome.out.bam -o $sample.ribocode -f0_percent 0.01 The log still reported the same contents, which causes RiboCode to interrput

liuliuliu0304 commented 3 weeks ago

I got the same trouble

Saurabh-plantist commented 3 weeks ago

I am wondering if you heard anything from the developer about this message or figure out the solution.

Best

DooYal commented 2 weeks ago

I am wondering if you heard anything from the developer about this message or figure out the solution.

Best

metaplots -a gencode.ribo.anno -r $sample.Aligned.toTranscriptome.out.bam -o $sample.ribocode -pv1 1 -pv2 1, the higher p value cut-offs possibly output some results