heatmap generate error

[zhanghxzll]

您好， 我在运行diting的最后生成热图时出现如下错误提示： 2022-05-26 13:56:34 [INFO ] **[8/12] Merge KEGG annotations* 2022-05-26 13:56:34 [INFO ] Merge KEGG annotations 2022-05-26 13:58:06 [INFO ] [9/12] Merge KEGG annotations and gene relative abundances 2022-05-26 13:58:06 [INFO ] Merge abundance table with kegg table 2022-05-26 13:59:20 [INFO ] Produce table of ko abundance among samples 2022-05-26 13:59:31 [INFO ] A table of ko abundance among samples was produced to diting_result/KEGG_annotation/ko_abundance_among_samples.tab 2022-05-26 13:59:31 [INFO ] ****[9/12] Build gene families**** 2022-05-26 13:59:31 [INFO ] Build gene family according to diting_result/KEGG_annotation/ko_abun.txt 2022-05-26 14:01:23 [INFO ] *[10/12] Generate hierarchical table**** 2022-05-26 14:01:23 [INFO ] *[11/12] Calculate abundance of pathways** 2022-05-26 14:01:27 [INFO ] ***[12/12] Diagrammatic drawing*** 2022-05-26 14:01:27 [INFO ] Visualize abundance table /home/zhengpf/.conda/envs/diting-env/bin/scripts/sketch.py:62: MatplotlibDeprecationWarning: normalize=None does not normalize if the sum is less than 1 but this behavior is deprecated since 3.3 until two minor releases later. After the deprecation period the default value will be normalize=True. To prevent normalization pass normalize=False
plt.pie(abundance_nor, colors=colors, startangle=90) Traceback (most recent call last): File "/home/zhengpf/.conda/envs/diting-env/bin/diting.py", line 255, in main() File "/home/zhengpf/.conda/envs/diting-env/bin/diting.py", line 239, in main sketch(ABUNDANCE_TABLE) File "/home/zhengpf/.conda/envs/diting-env/bin/scripts/sketch.py", line 450, in sketch color = colors[i-1] IndexError: list index out of range

运行脚本如下： diting.py -r /backup/diting/fq -a /backup/diting/contig_layer -o diting_result -n 40 -m 200 运行到最后可以产生pathways_relative_abundance.tab文件，但是无法生成图片，请问您可以看出是什么问题吗？ 谢谢！

[xuechunxu]

您好，

是不是因为样本数太多了啊？超过24个就不可以了，你可以根据需要合并你的样本，然后使用此命令生成图片：diting.py -vis <pathways_relative_abundance.tab>。

[zhanghxzll]

感谢回复！将样本数合并后确实可以了，但是之前报错是21个，也无法产生heatmap。