I have studied your paper recently and I think It's a great tool to estimate cell proportions.But some questions puzzled me，I wonder if I can ask you?
1.When starts from scRNA-seq data from multiple subjects,the immune cells(eg.B cell,CD4+ cells etc.)from peripheral blood mononuclear cells are the same to tumour-infiltrating immune cells?
2.As you show in the overview of MuSiC framework,calculates both cross-subject mean and cross-subject variance for these genes in each cell type，why select the informative gene only use cross-subject variance ?Can only use these low cross-subject variance genes identifies different cell types?
dear Dr.xuranw,
I have studied your paper recently and I think It's a great tool to estimate cell proportions.But some questions puzzled me，I wonder if I can ask you? 1.When starts from scRNA-seq data from multiple subjects,the immune cells(eg.B cell,CD4+ cells etc.)from peripheral blood mononuclear cells are the same to tumour-infiltrating immune cells? 2.As you show in the overview of MuSiC framework,calculates both cross-subject mean and cross-subject variance for these genes in each cell type，why select the informative gene only use cross-subject variance ?Can only use these low cross-subject variance genes identifies different cell types?
Best wishes, huitingxiao