Hi, I'm sorry if this is not the place but I cannot find any other resources. The MuSiC deconvolution tutorial is in 2 parts. One is where they deconvolve pancreatic cells using the music_prop function and the other where they deconvolve kidney cells, but in this case, they first cluster the cells using hclust and music_basis and then use music_prop.cluster to deconvolve the cells. I do not understand the point of this. Could someone please explain this part? I have been unable to find explanations of these steps.
Why didn't we cluster the cells in the example of the pancreatic cell or why was the process for kidney cells so much more elaborate than the pancreatic cells?
Hi, I'm sorry if this is not the place but I cannot find any other resources. The MuSiC deconvolution tutorial is in 2 parts. One is where they deconvolve pancreatic cells using the music_prop function and the other where they deconvolve kidney cells, but in this case, they first cluster the cells using hclust and music_basis and then use music_prop.cluster to deconvolve the cells. I do not understand the point of this. Could someone please explain this part? I have been unable to find explanations of these steps. Why didn't we cluster the cells in the example of the pancreatic cell or why was the process for kidney cells so much more elaborate than the pancreatic cells?
Sorry, if this is a very basic question.