Closed Puriney closed 7 years ago
strand="rev" is needed when counting UMIs. This is requested by internal users.
strand="rev"
2 possible solutions:
demultiplexing
count-umi
2nd is favored as it will have wider scope.
Solution Refs:
strand="rev"
is needed when counting UMIs. This is requested by internal users.2 possible solutions:
demultiplexing
. Trans-rev the R2.fastq reads before writing to demultiplexed reads files. Advantage is that the rest pipeline is untouched at all.count-umi
. Addstrand="rev"
when iterating each read. Context is here And probably adapting these 7 lines to our pipeline will solve the issue.2nd is favored as it will have wider scope.
Solution Refs: