yancylau / camelid_hcab

Script for the NGS data analysis of the Bactrian camel immune repertoires
1 stars 0 forks source link

文章方法咨询 #1

Open zhendejuzi opened 5 days ago

zhendejuzi commented 5 days ago

你好, 在文章方法的这一部分: 【Sequence annotation】 Variable regions, including FRs and CDRs were inferred using IgBLAST (Ye et al., 2013). Next, we numbered the gene sequences according to the IMGT numbering criteria (Lefranc et al., 2003). To determine the IGHV gene family, reference database was built from human germline IGHV genes in IMGT/GENE-DB (version: 202129-7) (Giudicelli et al., 2005). To determine the usage of Bactrian camel genes, reference database was built from Bactrian camel germline IGHV, IGHD, and IGHJ genes (Table S4) (Ming et al., 2020). IGHC genes were inferred based on the sequence of hinge regions.

在这里,我也想用IgBLAST,但是要如何找到IGHD和IGHJ的序列呢?我在IMGT上没有找到。请问数据是在哪里找的呢?

yancylau commented 2 days ago

Here is the directory for the IMGT reference database. https://www.imgt.org/vquest/refseqh.html