yarden / MISO

MISO: Mixture of Isoforms model for RNA-Seq isoform quantitation
http://genes.mit.edu/burgelab/miso/index.html
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sashimi_plot with index_gff --compressed-id parameter #114

Open genetalks-fred opened 7 years ago

genetalks-fred commented 7 years ago

index_gff run with --compressed-id parameter, but when using sashimi_plot scripts, 'can't fint pickle file happend', as followed:

commond: sashimiplot --plot-event misocomp-3865436414453376553 data/bioit/biodata/mengf/Project/Penile_cancer/GRCh37-results/Analysis-20170614/Altersplicing/Filt_trans_protein/generate_events/commonshortest/SE sashimi_plot_settings_len100_SE.txt --output-dir events

Traceback (most recent call last): File "/usr/local/bin/sashimi_plot", line 9, in load_entry_point('misopy==0.5.3', 'console_scripts', 'sashimi_plot')() File "/usr/local/lib/python2.7/dist-packages/misopy/sashimi_plot/sashimi_plot.py", line 276, in main plot_label=plot_label) File "/usr/local/lib/python2.7/dist-packages/misopy/sashimi_plot/sashimi_plot.py", line 142, in plot_event %(event_name, pickle_dir)

Exception: Event misocomp_-3865436414453376553 not found in pickled directory /data/bioit/biodata/mengf/Project/Penile_cancer/GRCh37-results/Analysis-20170614/Altersplicing/Filt_trans_protein/generate_events/commonshortest/SE. Are you sure this is the right directory for the event?

Use events raw id substitute compressed id, plot pdf was made with 'WARNING: MISO file not found' Any solution can solve this problem?

Thanks for help!