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MISO: Mixture of Isoforms model for RNA-Seq isoform quantitation
http://genes.mit.edu/burgelab/miso/index.html
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MISO Output error #122

Open ibmuller opened 6 years ago

ibmuller commented 6 years ago

Hello,

I recently did a run of MISO but I don't really get an output. It makes all the .miso files but the content shows no PSI values. I get an index error when I try to summarize which i assume is related.

Below are a batch log and an example .miso file.

I just used my own single end, STAR-aligned, indexed and sorted BAM file for input. with the hg19 GFF3 file provided by MISO for SE. I use Ensembl annotation for everything.

batch-5-07-11-18_13%3A41%3A09.log

batch-5-07-11-18_13%3A41%3A09.log

Any thoughts?

ibmuller commented 6 years ago

chr18%3A75849228%3A75849581%3A-@chr18%3A75847590%3A75847738%3A-@chr18%3A75838319%3A75838689%3A-.zip

here is a .miso file btw.

it simply doesnt seem to generate any PSI values.