yarden / MISO

MISO: Mixture of Isoforms model for RNA-Seq isoform quantitation
http://genes.mit.edu/burgelab/miso/index.html
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install error and plot error #146

Open jingydz opened 1 year ago

jingydz commented 1 year ago

install error log

$ pip install misopy Requirement already satisfied: misopy in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (0.5.4) Requirement already satisfied: scipy>=0.9.0 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from misopy) (1.10.0) Requirement already satisfied: pysam>=0.6.0 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from misopy) (0.17.0) Requirement already satisfied: matplotlib in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from misopy) (3.6.3) Requirement already satisfied: numpy>=1.5.0 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from misopy) (1.23.5) Requirement already satisfied: cycler>=0.10 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (0.11.0) Requirement already satisfied: pillow>=6.2.0 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (9.0.1) Requirement already satisfied: packaging>=20.0 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (21.3) Requirement already satisfied: pyparsing>=2.2.1 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (3.0.4) Requirement already satisfied: python-dateutil>=2.7 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (2.8.2) Requirement already satisfied: contourpy>=1.0.1 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (1.0.7) Requirement already satisfied: fonttools>=4.22.0 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (4.25.0) Requirement already satisfied: kiwisolver>=1.0.1 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from matplotlib->misopy) (1.3.2) Requirement already satisfied: six>=1.5 in /Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages (from python-dateutil>=2.7->matplotlib->misopy) (1.16.0) $ miso Traceback (most recent call last): File "/Parastor300s_G30S/zhangjj/software/miniconda3/bin/miso", line 5, in from misopy.miso import main File "/Parastor300s_G30S/zhangjj/software/miniconda3/lib/python3.9/site-packages/misopy/miso.py", line 62 print "MISO (Mixture of Isoforms model)" ^ SyntaxError: invalid syntax

plot error log

AS]$ rmats2sashimiplot --b1 13.bam,17.bam,9.bam,11.bam,19.bam --b2 1.bam,3.bam,7.bam,5.bam,20.bam -o rmats2sashimiplot_out -c chrX:+:67544021:67730619:/Parastor300s_G30S/zhangjj/genome/hg38/gencode.v40.annotation.gff3 --l1 delP4.post --l2 delP4.pre --exon_s 1 --intron_s 5

'13.bam' seems to be indexed already. Please Check out this index file '13.bam.bai' '17.bam' seems to be indexed already. Please Check out this index file '17.bam.bai' '9.bam' seems to be indexed already. Please Check out this index file '9.bam.bai' '11.bam' seems to be indexed already. Please Check out this index file '11.bam.bai' '19.bam' seems to be indexed already. Please Check out this index file '19.bam.bai' '1.bam' seems to be indexed already. Please Check out this index file '1.bam.bai' '3.bam' seems to be indexed already. Please Check out this index file '3.bam.bai' '7.bam' seems to be indexed already. Please Check out this index file '7.bam.bai' '5.bam' seems to be indexed already. Please Check out this index file '5.bam.bai' '20.bam' seems to be indexed already. Please Check out this index file '20.bam.bai'

'<' not supported between instances of 'int' and 'str' There is an exception in plot_with_coordinate