Closed rprechelt closed 6 years ago
We need to develop an autofocus routine to be called during queue startup to ensure good focus for the following imaging.
See discussion and use of Laplacion (LoG) filters here. Also kernel discussion [here](https://en.wikipedia.org/wiki/Kernel_(image_processing). Initial hacky attempts using LoG filter shows promising results.
Initial function layout here. Implementation should be confined to that file.
evaluate() should return the effective focus of the image using LoG filters, and then scipy's Newton's Method can be called to automatically optimize the focus function.
evaluate()
scipy
We need to develop an autofocus routine to be called during queue startup to ensure good focus for the following imaging.
See discussion and use of Laplacion (LoG) filters here. Also kernel discussion [here](https://en.wikipedia.org/wiki/Kernel_(image_processing). Initial hacky attempts using LoG filter shows promising results.
Initial function layout here. Implementation should be confined to that file.
evaluate()should return the effective focus of the image using LoG filters, and thenscipy's Newton's Method can be called to automatically optimize the focus function.