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yfukai / m2stitch

MIST-inspired microscope image stitching package
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Multiple channels #425

Open smith6jt-cop opened 8 months ago

smith6jt-cop commented 8 months ago

Hello!

Thank you once again for your work here. I am working on application of m2stitch to multiplex data with 29 channels, each 4-channel cycle has DAPI to register to other cycles, so I was wondering if the tile positions can be re-used for stitching other channels? MIST has this function to use the global-positions from one stitch for another so it would be nice to first stitch DAPI and then reuse the positions for the other 3 channels in the cycle. If you have another method for stitching multiple channels please let me know.

Thanks!

yfukai commented 8 months ago

Hi, thanks @smith6jt-cop for your interest! It is definitely possible to reuse the tile positions for different channels, since M2stitch is designed to return the stitched positions of the tiles. For example, you can use your DAPI images as images in the example, and rewrite the lines after # stitching example to stitch the images for all channels. I hope this helps!