search

yhwu / idemp

Barcode demultiplex for Illumina I1, R1, R2 fastq.gz files
GNU General Public License v2.0
30 stars 8 forks source link

Something wrong (different number of reads written and reads that should be written) #7

Closed nailabc closed 6 years ago

nailabc commented 6 years ago

Hi, I am performing the demultiplex for a Illumina Miseq 16S rRNA run (V4 region, paired reads) and I got a warning message like the one below for all my samples. It seems that it was not possible to write the correct number of reads per barcode. Any thoughts of why this could be happening?

5 reads should be written to /Users/Naila/Desktop/16S_glyphosate/demultiplex2/Undetermined_S0_L001_R1_001_out.fastq_negative.control.fastq.gz 2 reads were written

yhwu commented 6 years ago

without a piece of your data, it's tell what's the cause.

nailabc commented 6 years ago

In fact the input files had a problem. I solved it, thanks!