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yhwu / idemp

Barcode demultiplex for Illumina I1, R1, R2 fastq.gz files
GNU General Public License v2.0
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Output Files #8

Open emilyjunkins opened 6 years ago

emilyjunkins commented 6 years ago

My output files are *.unsigned.fastq and I1.decode. These are not described in to documentation. Are the unsigned files those that did not get paired with a barcode? If so, these are all my reads and how could I fix this?

idemp -b barcodes.all.csv -I1 index.fastq -R1 read1.fastq -R2 read.fastq

I ran this with out the -o command as I kept getting an error with mkdir

yhwu commented 6 years ago

Not paired. Probably low quality, or something wrong with barcode file.