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yu-lab-vt / AQuA-Fiji

AQuA Fiji plugin
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Get different result in MATLAB and FIJI #2

Open ZiTao956 opened 1 year ago

ZiTao956 commented 1 year ago

Thank you for this great work! I find out I will get different results in MATLAB and FIJI, the dF/F detected in MATLAB is unusually small.

Both AQuA is the newest version in MATLAB R2021b and FIJI, I chose the first option and set 0.33 sec per frame, 5 intensity threshold scaling factor. All the other parameters are default. I ran all the steps but ignore delay Tau. In MATLAB it detects 970 ROA, the dF/F range from 2.7519e-0.5 to 0.0045, while in FIJI it detects 463 ROA, dF/F range from 0.057 to 0.639. I find out MATLAB will show an error when I run AQuA GUI, but everything still works fine.

MATLAB result FIJI result Error in MATLAB

I attach my test file here: https://drive.google.com/file/d/1Gy_g7mBvYVIX6ftrL8TSXgV3Sa4ANVa9/view?usp=sharing

XuelongMi commented 1 year ago

Thank you for reminding us. We take the Matlab version as the standard. We've tried to find the difference between Fiji and Matlab version and modified the Fiji code. Now the newest release of Fiji would give a similar result.