search

yu-lab-vt / AQuA

An event-based tool for analyzing and quantifying calcium or neurotransmitter imaging data
51 stars 14 forks source link

Get different result in MATLAB AQuA and FIJI AQuA #16

Closed ZiTao956 closed 1 year ago

ZiTao956 commented 1 year ago

Thank you for this great work! I find out I will get different results in MATLAB and FIJI, the dF/F detected in MATLAB is unusually small.

Both AQuA is the newest version in MATLAB R2021b and FIJI, I chose the first option and set 0.33 sec per frame, 5 intensity threshold scaling factor. All the other parameters are default. I ran all the steps but ignore delay Tau. In MATLAB it detects 970 ROA, the dF/F range from 2.7519e-0.5 to 0.0045, while in FIJI it detects 463 ROA, dF/F range from 0.057 to 0.639. I find out MATLAB will show an error when I run AQuA GUI, but everything still works fine.

MATLAB result FIJI result Error in MATLAB

I attach my test file here: https://drive.google.com/file/d/1Gy_g7mBvYVIX6ftrL8TSXgV3Sa4ANVa9/view?usp=sharing

XuelongMi commented 1 year ago

Hi ZiTao, thank you for reminding us. The two versions show different results because the MATLAB version is the most updated, and some modifications are not synchronized on Fiji version. The MATLAB version is the version we continue improving.