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yu-lab-vt / AQuA2

Activity Quantification and Analysis for molecular spatiotemporal signals
GNU General Public License v2.0
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Multiple cell evaluation #9

Open Agrajag1984 opened 1 week ago

Agrajag1984 commented 1 week ago

I am rather new to Ca-imaging, but i have a cell culture with several cells yet i want to treat them individually. is there a way to analyse them with Aqua2 in one run or do I have to load the same tif for each cell and exclude the other cells via ROI selection? also i am not sure about the downstream analysis, as all detected traces look mostly the same in my tif i dont know, whether different peaks in the same ROI will get a new event (but then why are the other events also shown in the event curve?) is there a walk through video maybe that could clarify the entire process?

XuelongMi commented 1 week ago

Hi,

In the top-left corner of the GUI, you can click the "+" button to draw ROI. You can draw multiple ROIs before starting the analysis. AQuA2 will then detect events only within the ROIs you've selected during a single run.

Different peaks within the same ROI will be detected as separate events. However, because the event curve represents the average intensity across the entire dataset, events that share the same spatial footprint may have similar curves. Keep in mind that the event curve is just one way to quantify the event. AQuA2 also provides additional features, such as the time points when events begin and end. Or in the GUI, overlays of different colors will show the spatiotemporal features of the events.

Currently, we don't have a video tutorial available for this process, we’ll keep it in mind for the future. For now, you can refer to our paper for a detailed explanation.