Multimodal Reference mapping error using the MapQuery ()

[Ewura-Esi]

I am trying to reanalyze the dataset used in your paper using the code provide from the tutorial. I get this warning anytime I use the MapQuery function. Kindly see the code below and the warning outcome

pbmc <- MapQuery(

• anchorset = anchors,
• query = pbmc,
• reference = reference,
• refdata = list(
• celltype.l1 = "celltype.l1",
• celltype.l2 = "celltype.l2",
• predicted_ADT = "ADT"
• ),
• reference.reduction = "spca",
• ) Finding integration vectors Finding integration vector weights 0% 10 20 30 40 50 60 70 80 90 100% [----|----|----|----|----|----|----|----|----|----| **| Predicting cell labels Warning: Layer counts isn't present in the assay object; returning NULL Predicting cell labels Warning: Feature names cannot have underscores ('_'), replacing with dashes ('-') Warning: Layer counts isn't present in the assay object; returning NULL Transfering 228 features onto reference data predicted_ADT already present in query. Storing as predicted_predictedADT Warning: Layer counts isn't present in the assay object; returning NULL Warning: Layer counts isn't present in the assay object; returning NULL Warning: Layer counts isn't present in the assay object; returning NULL | | 0 % ~calculating
  Integrating dataset 2 with reference dataset Finding integration vectors Integrating data |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=03s
  Warning message: Key 'predictedADT' taken, using 'predictedpredictedadt_' instead

[yuchaojiang]

It seems that you've already transferred the labels and the slot predicted_ADT has been taken. This step is to infer the cell types of the PBMC multiomics data from an existing reference, and is not the core for TRIPOD.