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yuehuang2023 / cryoNeFEN

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The data preprocessing error for the 10076 dataset. #5

Open Rich-XGK opened 2 months ago

Rich-XGK commented 2 months ago

Dear Author,

I sincerely apologize for the continued disturbance, and I hope you can forgive me!

Following the instructions in your readme file, we attempted to process the 10076 dataset (1. import particles; 2. run an ab initio reconstruction job), keeping all settings as default. Below is a screenshot of our job page:

截屏2024-09-09 10 57 00 截屏2024-09-09 10 57 37 截屏2024-09-09 10 57 50

In the "ab initio reconstruction job," we encountered the following error. We would like to ask if you experienced this issue when processing this dataset, and how it was resolved. Thank you very much!

截屏2024-09-09 10 38 22
luluppang commented 2 months ago

I have the same error. Did you solve the problem? Thank you!

yuehuang2023 commented 2 months ago

Inverting the data during 'import' job may solve the problem

Rich-XGK commented 2 months ago

iwEcAqNwbmcDAQTRBjAF0QIQBrC7DFl4SWmWCgbJZng0otEAB9In_jd1CAAJomltCgAL0gADb1k png_720x720q90

截屏2024-09-11 11 36 30 截屏2024-09-11 11 38 03

As shown in the figure, I used .star and .mrc files as inputs for the "Import Particles" job to import the particles and set the "Data Sign" to "dark-on-light" to invert the data. However, I still encountered the aforementioned issue in the Ab-Initio Reconstruction job.