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yukiteruono / pbsim3

PBSIM3: a simulator for all types of PacBio and ONT long reads
GNU General Public License v2.0
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About the max length of simulated HIFI read #1

Open 11malong opened 1 year ago

11malong commented 1 year ago

For HIFI read generated, what is the max length of HIFI read?

yukiteruono commented 1 year ago

Thank you for your using PBSIM3. The maximum HiFi read length is 1 million. If you get an error message "ERROR: length parameters are not appropriate", the generation of read length distribution is not working properly, so please change the value of --length-sd. For multi-pass sequencing in WGS simulation, the read length is set roughly equal to the value of --length-mean, and --length-sd is disabled. There was a bug in the --length-mean check, which has now been fixed.

11malong commented 1 year ago

Thank you so much!