search

z0on / tag-based_RNAseq

Cost-efficient genome-wide gene expression profiling
16 stars 9 forks source link

multiple template switching oligos? #2

Closed onecarbon closed 4 years ago

onecarbon commented 4 years ago

Hi

Thanks for this - I am interested in trying it out! Could you clarify the reason for the multiple template switching oligos in this version of the protocol? It looks like the published versions have only one? Are all three now recommended?

Thanks

z0on commented 4 years ago

Hello! Three oligos are needed to diversify base composition near beginning of the read, which is very important for latest models of illumina sequencers.

iPhone. Apologies for brevity

On Nov 5, 2019, at 4:21 PM, finnhamilton notifications@github.com wrote:

Hi

Thanks for this - I am interested in trying it out! Could you clarify the reason for the multiple template switching oligos in this version of the protocol? It looks like the published versions have only one? Are all three now recommended?

Thanks

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub, or unsubscribe.

onecarbon commented 4 years ago

Thanks! will try that way then