Normalizing graphs by total island filitered reads per million and generating summary WIG file...
Removing temporary directory and all files in it.
Traceback (most recent call last):
File "/usr/local/apps/sicer/2-1.0.0/bin/sicer", line 231, in <module>
main()
File "/usr/local/apps/sicer/2-1.0.0/bin/sicer", line 225, in main
run_SICER.main(args)
File "/usr/local/Anaconda/envs_app/sicer2/1.0.0/lib/python3.6/site-packages/sicer/main/run_SICER.py", line 73, in main
make_normalized_wig.main(args, output_WIG_name, pool)
File "/usr/local/Anaconda/envs_app/sicer2/1.0.0/lib/python3.6/site-packages/sicer/src/make_normalized_wig.py", line 46, in main
with open(outfile_path, 'w') as outfile:
FileNotFoundError: [Errno 2] No such file or directory: 'sicer_out/test-W200-normalized.wig'
Running without the "-o" parameter (i.e. sicer -t test.bed -c ctrl.bed -s mm10 -cpu 16) gives output and my job runs to completion.
Tried running with the following commands (also had to specify .bed inputs, as I'm also getting the same bamtobed issue reported here):
sicer -t test.bed -c ctrl.bed -s mm10 -cpu 16 -o sicer_out
Running without the "-o" parameter (i.e. sicer -t test.bed -c ctrl.bed -s mm10 -cpu 16) gives output and my job runs to completion.