Can you please help me understand how are the "-m" flag and "NC" related. To my knowledge, both relate to the # of soft clipped required at the breakpoint. I am using -m flag (minimum number of soft-clips supporting START) as 15 while running WHAM but I see that most of my calls still have NC < 15. It shouldn't actually report any SV that has soft clips collapsed into the consensus that are less than 15. Can you please clarify this one for me.
Hi Zev,
Can you please help me understand how are the "-m" flag and "NC" related. To my knowledge, both relate to the # of soft clipped required at the breakpoint. I am using -m flag (minimum number of soft-clips supporting START) as 15 while running WHAM but I see that most of my calls still have NC < 15. It shouldn't actually report any SV that has soft clips collapsed into the consensus that are less than 15. Can you please clarify this one for me.
Thanks for your help.
Ashini