[E::bgzf_read] Read block operation failed with error 4 after 0 of 4 bytes samtools view: error reading file "HiC.bam" samtools view: error closing "HiC.bam": -1

[cht-jk]

Hello author： （1）bwa mem -5SP -t 80 Six_genome.bp.p_ctg.fasta Q-6-Hi_c_1.clean.fq.gz Q-6-Hi_c_2.clean.fq.gz | samblaster | samtools view - -@ 80 -S -h -b -F 3340 -o HiC.bam （2）/home/export/online3/caohaitao/HapHiC/haphic/HapHiC/utils/filter_bam HiC.bam 1 --nm 3 --threads 80 | samtools view - -b -@ 80 -o HiC.filtered.bam The following error occurred while running step (2): [E::bgzf_read] Read block operation failed with error 4 after 0 of 4 bytes samtools view: error reading file "HiC.bam" samtools view: error closing "HiC.bam": -1

[zengxiaofei]

It seems that your HiC.bam was not correctly generated. Please check the file using samtools view HiC.bam

[cht-jk]

haphic pipeline Six_genome.bp.p_ctg.fasta HiC.filtered.bam 25 --quick_view --RE "GATC" --correct_nrounds 2 --remove_allelic_links 4 --threads 60 --processes 60 Will the information indicated in the figure have an impact on the results?

[zengxiaofei]

The sparse_dot_mkl module is only used to speed up the clustering step. It does not affect the results. --remove_allelic_links 4 is used to deal with haplotype-resolved autotetraploid assemblies. As your input assembly file is p_ctg and the chromosome number is 25, this parameter is not necessary for your case.

[cht-jk]

haphic reassign ../Six_genome.bp.p_ctg.fasta full_links.pkl mcl_inflation_4.2.clusters.txt paired_links.clm --nclusters 25 Running Reassign alone resulted in the following error thanks！

[zengxiaofei]

FileNotFoundError

[cht-jk]

Hello, I would like to use only Haphic to draw the HIC heatmap. Can I skip the mounted part and only obtain the HIC heatmap without changing the original genome?

[zengxiaofei]

Yes, for more information, please see our note in the Visualization section.

[cht-jk]

Hello, thank you for your answer. Currently, I am only using Haphic to draw a heatmap and skipping mounting. The resulting heatmap does not meet expectations. What is the situation?

[zengxiaofei]

1. How did you align the Hi-C reads to the assembly and filter them?
2. Does the sixth column of the AGP file match the reference names of the BAM file for haphic plot?
3. Could you please show me the commands you used?

[cht-jk]

（1） $bwa mem -5SP -t 50 sweettea.racon.2.fa R._c_var._suavissimus-3_1.clean.fq.gz R._c_var._suavissimus-3_1.clean.fq.gz | samblaster | samtools view - -@ 50 -S -h -b -F 3340 -o HiC.bam $filter_bam HiC.bam 1 --nm 3 --threads 35 | samtools view - -b -@ 35 -o HiC.filtered.bam $mock_agp_file.py sweettea.racon.2.fa > sweettea.agp $haphic plot sweettea.agp HiC.filtered.bam --bin_size 1000 --min_len 5 --threads 80 （2） does the sixth column of the AGP file match the reference name of the BAM file exactly

[zengxiaofei]

You used the same file R._c_var._suavissimus-3_1.clean.fq.gz for both ends of the Hi-C reads.

[cht-jk]

Oh, I just realized. Thank you very much

[cht-jk]

How to split group3 into two parts, one green part named group3 and the other green part named group26，thanks.

[zengxiaofei]

https://github.com/aidenlab/Juicebox https://github.com/zengxiaofei/HapHiC#juicebox-curation