zhangaidi1010 / ea-utils

Automatically exported from code.google.com/p/ea-utils
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fastq-multx #11

Closed GoogleCodeExporter closed 8 years ago

GoogleCodeExporter commented 8 years ago
What steps will reproduce the problem?
1.i want to use the fastq-multx for demultiplexing the barcode file with the 
reads file i am using this command
fastq-multx -b barcode.txt s1.fq s2.fq -o 1%.fq -o 2%.fq

2.
3.

What is the expected output? What do you see instead?
i need to create two output files with barcodes in it

What version of the product are you using? On what operating system?
ea-utils.1.1.2-484 i am using in mac

Please provide any additional information below.
can i get steps how to run this command in the command line

i just downloaded it and trying to install

Original issue reported on code.google.com by abhina...@gmail.com on 10 Nov 2012 at 12:09

GoogleCodeExporter commented 8 years ago
I can't tell from your example what the design is, but if the barcode read is 
in a separate file :

SINGLE_END:
fastq-multx -B barcode-definition.txt BARCODE_READ.fq.gz REGULAR_READ.fq.gz -o 
n/a -o OUT.%.fq.gz

PAIRED_END:
fastq-multx -B barcode-definition.txt BARCODE_READ.fq.gz REGULAR_READ1.fq.gz 
REGULAR_READ2.fq.gz -o n/a -o OUT.%.1.fq.gz -o OUT.%.2.fq.gz

Original comment by earone...@gmail.com on 6 Dec 2012 at 5:04

GoogleCodeExporter commented 8 years ago

Original comment by earone...@gmail.com on 12 Feb 2013 at 6:32