RunSCVELO error

[qindan2008]

I used the latest version of SCP(v0.3.0), error still occurred:

[zhanghao-njmu]

SCP (v0.3.0) is not the latest version. You need to update SCP and try again.

[Byronxy]

Hi, I get a different error

> pancreas_sub <- RunSCVELO(
+     srt = pancreas_sub, group_by = "SubCellType",
+     linear_reduction = "PCA", nonlinear_reduction = "UMAP"
+ )
'misc' slot is not converted.
'tools' slot is not converted.
Error: RuntimeError: numpy.linalg.LinAlgError: Singular matrix

I used the latest version of SCP

[qindan2008]

I updated SCP to version 0.4.7.9000, and still got the same error

[DocMonster77]

pancreas_sub <- RunPAGA(srt = pancreas_sub, group_by = "SubCellType", linear_reduction = "PCA", nonlinear_reduction = "UMAP") 'misc' slot is not converted. 'tools' slot is not converted. Error in match.arg(arg = layer, choices = Layers(object = object, search = FALSE)) : 'arg' should be one of "counts", "data", "scale.data"

[DocMonster77]

I get a different error pancreas_sub <- RunSCVELO(

• srt = pancreas_sub, group_by = "SubCellType",
• linear_reduction = "PCA", nonlinear_reduction = "UMAP"
• ) 'misc' slot is not converted. 'tools' slot is not converted. Filtered out 12123 genes that are detected 30 counts (shared). Normalized count data: X, spliced, unspliced. Logarithmized X. computing neighbors finished (0:00:00) --> added 'distances' and 'connectivities', weighted adjacency matrices (adata.obsp) computing moments based on connectivities finished (0:00:01) --> added 'Ms' and 'Mu', moments of un/spliced abundances (adata.layers) computing velocities finished (0:00:00) --> added 'stochastic', velocity vectors for each individual cell (adata.layers) computing velocity graph (using 1/12 cores) Error in match.arg(arg = layer, choices = Layers(object = object, search = FALSE)) : 'arg' should be one of “counts”, “data”, “scale.data”

[zjh0613]

Hello developers, when I run RunSCVELO I get an error. How did this happen and how do I fix it. Thank you Next is the basic information I entered for the seruat object