RStudio crashed when executing RunPAGA() function

[whiteSongLin]

Error occurred while I was following the provided tutorial. Is this step memory-intense? My MacBook is 8G RAM only.

> sessionInfo()
R version 4.3.2 (2023-10-31)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Sonoma 14.2.1

Matrix products: default
BLAS:   /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/lib/libRlapack.dylib;  LAPACK version 3.11.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: Asia/Hong_Kong
tzcode source: internal

attached base packages:
[1] stats     graphics  grDevices datasets  utils     methods   base     

loaded via a namespace (and not attached):
  [1] fs_1.6.3                    matrixStats_1.2.0           spatstat.sparse_3.0-3      
  [4] bitops_1.0-7                enrichplot_1.22.0           HDO.db_0.99.1              
  [7] httr_1.4.7                  RColorBrewer_1.1-3          doParallel_1.0.17          
 [10] tools_4.3.2                 sctransform_0.4.1           utf8_1.2.4                 
 [13] R6_2.5.1                    HDF5Array_1.30.1            lazyeval_0.2.2             
 [16] uwot_0.1.16                 rhdf5filters_1.14.1         GetoptLong_1.0.5           
 [19] withr_2.5.2                 sp_2.1-3                    prettyunits_1.2.0          
 [22] gridExtra_2.3               progressr_0.14.0            cli_3.6.2                  
 [25] Biobase_2.62.0              spatstat.explore_3.2-7      fastDummies_1.7.3          
 [28] scatterpie_0.2.1            Seurat_5.0.3                spatstat.data_3.0-4        
 [31] ggridges_0.5.6              pbapply_1.7-2               slingshot_2.10.0           
 [34] Rsamtools_2.18.0            yulab.utils_0.1.2           gson_0.1.0                 
 [37] DOSE_3.28.2                 R.utils_2.12.3              parallelly_1.36.0          
 [40] RSQLite_2.3.4               generics_0.1.3              gridGraphics_0.5-1         
 [43] shape_1.4.6                 ica_1.0-3                   spatstat.random_3.2-3      
 [46] dplyr_1.1.4                 GO.db_3.18.0                Matrix_1.6-5               
 [49] fansi_1.0.6                 S4Vectors_0.40.2            abind_1.4-5                
 [52] R.methodsS3_1.8.2           lifecycle_1.0.4             SummarizedExperiment_1.32.0
 [55] rhdf5_2.46.1                qvalue_2.34.0               SparseArray_1.2.3          
 [58] BiocFileCache_2.10.1        Rtsne_0.17                  grid_4.3.2                 
 [61] blob_1.2.4                  promises_1.2.1              crayon_1.5.2               
 [64] miniUI_0.1.1.1              lattice_0.21-9              cowplot_1.1.2              
 [67] KEGGREST_1.42.0             pillar_1.9.0                ComplexHeatmap_2.18.0      
 [70] fgsea_1.28.0                GenomicRanges_1.54.1        rjson_0.2.21               
 [73] future.apply_1.11.1         codetools_0.2-19            fastmatch_1.1-4            
 [76] leiden_0.4.3.1              glue_1.6.2                  ggfun_0.1.3                
 [79] data.table_1.14.10          vctrs_0.6.5                 png_0.1-8                  
 [82] treeio_1.26.0               spam_2.10-0                 gtable_0.3.4               
 [85] cachem_1.0.8                princurve_2.1.6             Signac_1.13.0              
 [88] S4Arrays_1.2.0              mime_0.12                   tidygraph_1.3.0            
 [91] survival_3.5-7              SingleCellExperiment_1.24.0 RcppRoll_0.3.1             
 [94] SCP_0.5.6                   iterators_1.0.14            ellipsis_0.3.2             
 [97] fitdistrplus_1.1-11         ROCR_1.0-11                 nlme_3.1-163               
[100] ggtree_3.10.0               bit64_4.0.5                 progress_1.2.3             
[103] filelock_1.0.3              RcppAnnoy_0.0.22            GenomeInfoDb_1.38.5        
[106] R.cache_0.16.0              irlba_2.3.5.1               KernSmooth_2.23-22         
[109] colorspace_2.1-0            BiocGenerics_0.48.1         DBI_1.2.0                  
[112] tidyselect_1.2.0            proxyC_0.4.1                bit_4.0.5                  
[115] compiler_4.3.2              curl_5.2.0                  xml2_1.3.6                 
[118] DelayedArray_0.28.0         plotly_4.10.4               shadowtext_0.1.2           
[121] scales_1.3.0                lmtest_0.9-40               rappdirs_0.3.3             
[124] stringr_1.5.1               digest_0.6.33               goftest_1.2-3              
[127] spatstat.utils_3.0-5        XVector_0.42.0              htmltools_0.5.7            
[130] pkgconfig_2.0.3             MatrixGenerics_1.14.0       dbplyr_2.4.0               
[133] fastmap_1.1.1               rlang_1.1.2                 GlobalOptions_0.1.2        
[136] htmlwidgets_1.6.4           shiny_1.8.0                 farver_2.1.1               
[139] zoo_1.8-12                  jsonlite_1.8.8              BiocParallel_1.36.0        
[142] GOSemSim_2.28.0             R.oo_1.25.0                 RCurl_1.98-1.13            
[145] magrittr_2.0.3              GenomeInfoDbData_1.2.11     ggplotify_0.1.2            
[148] dotCall64_1.1-1             patchwork_1.1.3             Rhdf5lib_1.24.2            
[151] munsell_0.5.0               Rcpp_1.0.11                 TrajectoryUtils_1.10.1     
[154] ape_5.7-1                   ggnewscale_0.4.9            viridis_0.6.4              
[157] reticulate_1.35.0           stringi_1.8.3               ggraph_2.1.0               
[160] zlibbioc_1.48.0             MASS_7.3-60                 plyr_1.8.9                 
[163] parallel_4.3.2              listenv_0.9.0               ggrepel_0.9.4              
[166] deldir_2.0-2                Biostrings_2.70.1           graphlayouts_1.0.2         
[169] splines_4.3.2               tensor_1.5                  hms_1.1.3                  
[172] circlize_0.4.16             igraph_1.6.0                spatstat.geom_3.2-9        
[175] RcppHNSW_0.6.0              reshape2_1.4.4              biomaRt_2.58.0             
[178] stats4_4.3.2                XML_3.99-0.16               SeuratObject_5.0.1         
[181] renv_1.0.7                  foreach_1.5.2               tweenr_2.0.2               
[184] httpuv_1.6.13               RANN_2.6.1                  tidyr_1.3.0                
[187] purrr_1.0.2                 polyclip_1.10-6             future_1.33.1              
[190] clue_0.3-65                 scattermore_1.2             ggplot2_3.4.4              
[193] ggforce_0.4.1               xtable_1.8-4                RSpectra_0.16-1            
[196] tidytree_0.4.6              later_1.3.2                 viridisLite_0.4.2          
[199] tibble_3.2.1                clusterProfiler_4.10.0      aplot_0.2.2                
[202] memoise_2.0.1               AnnotationDbi_1.64.1        IRanges_2.36.0             
[205] cluster_2.1.4               globals_0.16.2