Hi,
I hope to apply ComBat_seq to my microbial otu table, my table has 214 cols and 6663 rows, but when I put it in the command below it return 'no data rows with required number of counts'. If there is anyone who could help me check with it, Is there something going wrong with my data? It's all numeric and have some 0 inside.
`> nrow(a)
[1] 6663
adjusted_counts <- ComBat_seq(a, batch=metadata$Souce, group=NULL, full_mod=FALSE)
Found 3 batches
Using null model in ComBat-seq.
Adjusting for 0 covariate(s) or covariate level(s)
Estimating dispersions
Error in dispCoxReid(y, design = design, offset = offset, subset = subset, :
no data rows with required number of counts`
Hi, I hope to apply ComBat_seq to my microbial otu table, my table has 214 cols and 6663 rows, but when I put it in the command below it return 'no data rows with required number of counts'. If there is anyone who could help me check with it, Is there something going wrong with my data? It's all numeric and have some 0 inside.
`> nrow(a) [1] 6663
I will appreciate any help!