Closed ttriche closed 7 years ago
Program: BISCUIT (BISulfite-seq CUI Toolkit) Version: 0.2.0.20161222
$ biscuit align hg38.fa -t16 -p -c POETIC-11_R1.fastq POETIC-11_R2.fastq > output.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1059604 sequences (160000204 bp)... [M::process] 1059604 single-end sequences; 0 paired-end sequences Floating point exception
$ biscuit align hg38.fa -t16 -c POETIC-11_R1.fastq POETIC-11_R2.fastq > output.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1059604 sequences (160000204 bp)... Floating point exception
$ biscuit align hg38.fa -t16 -p POETIC-11_R1.fastq POETIC-11_R2.fastq > output.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [W::main_align] when '-p' is in use, the second query file is ignored. [M::process] read 1059604 sequences (160000204 bp)... [M::process] 1059604 single-end sequences; 0 paired-end sequences [M::process] read 1059604 sequences (160000204 bp)... [M::mem_process_seqs] Processed 1059604 reads in 3742.793 CPU sec, 235.158 real sec [M::process] 1059604 single-end sequences; 0 paired-end sequences [M::process] read 1059604 sequences (160000204 bp)... [M::mem_process_seqs] Processed 1059604 reads in 3649.333 CPU sec, 228.816 real sec [M::process] 1059604 single-end sequences; 0 paired-end sequences [M::process] read 1059604 sequences (160000204 bp)... [M::mem_process_seqs] Processed 1059604 reads in 3642.387 CPU sec, 232.409 real sec Segmentation fault
$ biscuit align hg38.fa -p -c POETIC-11_R1.fastq POETIC-11_R2.fastq > output.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] 66226 single-end sequences; 0 paired-end sequences Floating point exception
$ biscuit align hg38.fa -c POETIC-11_R1.fastq POETIC-11_R2.fastq > output.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... Floating point exception
WGBS runs from a HiSeq X
Misuse of arguments on my part! Closing.
Program: BISCUIT (BISulfite-seq CUI Toolkit) Version: 0.2.0.20161222
WGBS runs from a HiSeq X