Conversion Rate calculation

zhou-lab / biscuit

BISulfite-seq CUI Toolkit

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Conversion Rate calculation #45

Closed Irfanwustl closed 3 years ago

Irfanwustl commented 3 years ago

Hi, Thanks for the great tool.

I have used BISCUIT and generated bw and bam files. However, I am trying to calculate the bisulfite conversion rate. Is there any way so that BISCUIT can provide the rate from the aligned bam file?

Or do I need to write a script? In the aligned bam file, I have found that there are ZC (Number of cytosine conversions) and ZR (Number of cytosine retentions) tags. Probably I need to use those to calculate the rate?

jamorrison commented 3 years ago

Hi @Irfanwustl

BISCUIT is now maintained at the huishenlab repo. If you are still in need of help, please open an issue on that repo's Issues page.

Irfanwustl commented 3 years ago

Thanks