I have used BISCUIT and generated bw and bam files. However, I am trying to calculate the bisulfite conversion rate. Is there any way so that BISCUIT can provide the rate from the aligned bam file?
Or do I need to write a script? In the aligned bam file, I have found that there are ZC (Number of cytosine conversions) and ZR (Number of cytosine retentions) tags. Probably I need to use those to calculate the rate?
Hi, Thanks for the great tool.
I have used BISCUIT and generated bw and bam files. However, I am trying to calculate the bisulfite conversion rate. Is there any way so that BISCUIT can provide the rate from the aligned bam file?
Or do I need to write a script? In the aligned bam file, I have found that there are ZC (Number of cytosine conversions) and ZR (Number of cytosine retentions) tags. Probably I need to use those to calculate the rate?