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zhouzilu / DENDRO

Genetic Heterogeneity Profiling by Single Cell RNA Sequencing
GNU General Public License v3.0
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10x scRNA-seq #14

Open doctorjb77 opened 3 years ago

doctorjb77 commented 3 years ago

Hello,

Thanks for this resource. I have a scRNA-seq dataset with close to 10,000 cells from droplet based sequencing (10x chromium). How does the pipeline handle large number of cells from droplet based sequencing?

Thanks! Brian

zhouzilu commented 3 years ago

Hi Brian, Unfortunately, from our research, 10X based (5' or 3') scRNA-seq don't work well as only a tiny portion of the genome will be captured of very low depth... Zilu