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zhpn1024 / ribotish

Ribo-seq TIS Hunter, predicting translation initiation sites and ORFs using riboseq data
http://dx.doi.org/10.1038/s41467-017-01981-8
GNU General Public License v3.0
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ribotish predict module run error #32

Closed haowBio closed 1 year ago

haowBio commented 1 year ago

Hellow when I run ribotish predict using stringtie assembly gtf which include new transcript info, it return this error image after removing new transcript it run successfully. How can I solve it?

This is my gtf image

zhpn1024 commented 1 year ago

The key problem is that the 'trans.strand' is '.', not '+' or '-'. Are there ways and options to assign strand in stringtie?

haowBio commented 1 year ago

The key problem is that the 'trans.strand' is '.', not '+' or '-'. Are there ways and options to assign strand in stringtie?

Thank for you reply After deleting the transcript which which strand is '.', It run successfully.