Could you please suggest correct parameters to generate WGS data with only sequencing error rate but no mutation rate? I mean I am trying to generate BAM file without any random mutations added due to mutation model, but still I need mutations from sequencing error rate/model?
Here, I mention -M 0 and -E 0.10 as I need muations from only sequencing error rate. But the golden VCF file does not have mutations added in. Any suggestions?
Hi,
Could you please suggest correct parameters to generate WGS data with only sequencing error rate but no mutation rate? I mean I am trying to generate BAM file without any random mutations added due to mutation model, but still I need mutations from sequencing error rate/model?
I tried this command :
python ~/software/neat-genreads/genReads.py -r ~/software/hg38/Chromosomes/chr22.fa -R 126 -o chr22_test12a --bam --vcf --pe 300 30 --rng 10 --no-fastq -c 30 -M 0 -E 0.10 -e ~/software/neat-genreads/models/errorModel_toy.p
Here, I mention -M 0 and -E 0.10 as I need muations from only sequencing error rate. But the golden VCF file does not have mutations added in. Any suggestions?
Thanks very much.