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Hi all, thanks for developing this very useful tool.
I have a question on how the clinvar variant significance annotation table is matched to the generated guides. I've attached a subset of my out…
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Hi, I encounter an error at the very last step during the design of sgRNA for CRISPR activation.
Everything is going fine until I try to add the CRISPRai scores.
After typing the command line in R…
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Hi,
I am trying to design sgRNA for campylobacter, but realised the campylobacter genome sequence is not available in the shiny app. Is there a way to upload my own genome sequence into the app? …
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This is urgent. I just tried meta-mode design guideRNA for human EMX1 and EGFR, but has error retrieving sgRNA sequence? Also there's no longer a clear / refresh button, is this removed intentionally?…
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### Description of the bug
The process NFCORE_CRISPRSEQ:CRISPRSEQ_SCREENING:MAGECK_FLUTEMLE starts the FluteMLE command using the data of the gene_summary.txt file. It downloads several pathway files…
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For some ENST numbers and HGNC we run into the error `AttributeError: 'str' object has no attribute 'iteritems'` (whole error at the bottom). This happens because sometimes the `edit_map` becomes an e…
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**Analysis of tested guide RNA’s used for gene editing via CRISPR to predict optimal future guide sequences**
Despite the apparent simplicity of this sequence matching strategy to target the CRISPR…
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[config_mApple2_gRNA_100919.txt](https://github.com/zhangchonglab/CRISPRi-functional-genomics-in-prokaryotes/files/3714579/config_mApple2_gRNA_100919.txt)
first of all, nice tool!. I have been tryi…
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I am trying to run getCrispraiScores using the vignette example, but run into the following error.
***Docker install:***
```
sudo docker run --rm -ti -p 8788:8787 -v $(pwd):/guide_design -e DISAB…
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Dear brocklab research group:
You did an excellent job in the field of lineage-tracing. Our research group is using the technology you developed to construct the ClonMapper library.In or…