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i tried poreseq consensus and i got the error:
Skipping FMH_MEDMicroBio_140808_S.typhi_H566_3440_1_ch110_file69_strand.fast5:18000:25780: file has no sequences defined (mode='rb') - is it SAM/BAM form…
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Based on the [Connor/doc/METHODS.rst](https://github.com/umich-brcf-bioinf/Connor/blob/master/doc/METHODS.rst) file, it looks like reads with identical UMTs are used to build consensus sequences. For …
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### [Transcription factors](https://www.youtube.com/watch?v=Sd3imcsvXxI)
1. Transcription factor (proteins) control the expression of genes by regulating the process of gene transcription.
![gene-…
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hi, i used racon to polish nanopore reads, the output file contains polished and unpolished reads, total number of polished and unpolished reads equal raw data, is it normal? for example, three reads…
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An idea raised in https://github.com/roblanf/sangeranalyseR/issues/46, to build consensus sequences while taking account of the phred scores. It's certainly possible.
Right now we use DECIPHER's t…
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Hi,
In MaSuRCA-3.2.7 the "Gap consensus" stage fails with the below error.
Not sure exactly how to fix this problem.
Thanks.
[Fri Aug 10 10:38:00 AEST 2018] Using linking mates
Using CABO…
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```
What steps will reproduce the problem?
1. Create New Project and Choose Location of Sequencing Files
2. Add Trace Files and then Add Trace Files to Project
3. Select Trace Files and then Generate …
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HI
I recently use RelocaTE2 to check TE insertion in my sample。 I put multiple consensus sequences in one fasta format to check TE insertion in one genome 。 Here is my output, i found tha NAME filed…
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Dear Wayne,
Every now and then I'm looking for freely available molecular biology software, e.g. to give it to our beginner students or use it by myself.
For my own little problems I'm actually u…
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Hi,
The script worked really well when I merged up to 255 loci from 196 species. However, I was having issue when the taxa number increased to 996, and the error message showed the process was kill…
ypoh1 updated
3 months ago