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Hi guys!
I'm trying to run small RNA-seq on Solanum tuberosum.
It did cutadapt, STAR, sambamba and then it hangs on
[mydir]/tools/bcbio/anaconda/bin/seqcluster cluster -o [mydir]/mirna/work/seqclu…
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Hi Brad,
just a quick question. Is there a reason you omitted some VEP annotation options during that step?
Wouldn't it be possible to just use the "--everything" option to maximize the annotations …
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ValueError: Cannot detect which reference version /usr/local/share/bcbio-nextgen/genomes/Hsapiens/GRCh37/bowtie2/GRCh37 is. Should end in either .ebwt (bowtie) or .bt2 (bowtie2).
It's clear that the …