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I'm trying to run your code and I saw the requirement is BLASR 2.0 but on the BLASR github page installing legacy BLASR isn't possible. So I installed the lastest version (BLASR 5.1) but that version …
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Hi all,
We are trying to get this pipeline working with the included control dataset so we can use it to characterize a variety of novel bacteriophages that we think may be circularly permuted with…
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Hi,
When trying the Pipecraft v.1.0.0 NextITS workflow I get the following error messages:
```
N E X T F L O W ~ version 23.04.1
Launching `/scripts/NextITS/Step2_AggregateRuns.nf` [admiring…
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Hi,
Can you please give a suggestion on how to use SEACR with experiments that have spike-in and replicates. What do you recommend as far as normalization with spike-in and how to handle replicates?
…
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If I have a CRAM file with `embed_ref=1` how do I get the reference out?
I can't find any `samtools` gateway to this, which I assume is probably in the `htslib` API ?
A use case for this is to f…
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We are close to releasing our new pipeline schema which describe input parameters. Through chatting to people at BOSC 🍐 I've become aware of a project called [Bioschemas](https://bioschemas.org) which…
ewels updated
3 years ago
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https://ming-lian.github.io/2019/08/19/Bioinfo-ML-Club-6th/
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Hi Pall,
Thanks for making pizzly. I've been working on adding support for it and some other fast fusion callers to bcbio and have been seeing if I can figure out some way of filtering the fusions …
roryk updated
6 years ago
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Hi,
I am new to programming and I am trying to run Unfazed to phase my DNMs, but could't make it
Here is my code:
`unfazed -d My_DNM_File.vcf -s Main_VCF_File_With_All_Variants.vcf.gz -p pedigr…
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Some questions
* Will FACS sorting disturb RNA?
* How should we preserve the cells post-sorting and pre-RNA isolation?
* How should we isolate RNA?
* What markers should we sort on?