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Hi team
Could you please share the manual with the possible command lines that could be used for analysis?
I kindly requested to have access to the website (GUI version) as I think it is much eas…
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Hi @FrederickHuangLin ,
Thanks for the great software! I am seen many references to using ANCOMBC on "raw data". I am working with shotgun metagenomes where raw reads are affected by gene length, …
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This what is written in Arguments explanation
a numerical fraction between 0 and 1. Taxa with prevalences less than prv_cut will be excluded in the analysis. Default is 0.10.
Is the taxa prevale…
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Hello Frederick,
Thanks again for this package and your hard work on improving it continuously. It has been essential for my PhD!
I saw that you have just submitted an update, so looking forward …
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Any idea what this error is?
```
> carbom
phyloseq-class experiment-level object
otu_table() OTU Table: [ 445 taxa and 4948 samples ]
sample_data() Sample Data: [ 4948 samp…
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ANCOMBC does currently support `phyloseq`. We are now actively working on an alternative framework - [miaverse](https://microbiome.github.io/) - based on the newer (Tree)SummarizedExperiment class, pr…
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I suppose this is more of a stats question than anything else, but I have been using ANCOM-BC and discovered that the vast majority of my differentially abundant taxa (q-val < 0.05) have a standard er…
eam12 updated
2 years ago
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Hi developer,
Does ANCOMBC support only one continuous variable of interest?
For example, in the vignettes:
> out = ancombc(phyloseq = phylum_data, formula = "age + nation + bmi_group",
> …
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I have with me the results from DADA2 analysis of 16S rRNA amplicon data (total of 139000 ASVs) from 44 control and 43 treated libraries. When I ran the below on ANCOMBC v1.2.2, I got only **1 ASV** d…
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Hello,
I am Ana, a Veterinary Student form Spain. I would like to compare the microbiome composition of sows given 4 different treatments, and see if there is any difference in the relative abundan…