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@golu099 brought up [GAPPadder](https://github.com/simoncchu/GAPPadder) as a tool to potentially replace [Gap2Seq](https://github.com/rikuu/Gap2Seq) for filling gaps in seq coverage between scaffolded…
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The Megakaryocyte_rep2 data is much smaller than the other samples. Is this intended? In https://www.ncbi.nlm.nih.gov/sra/?term=SRR549358 the original data of the two samples is about the same size. I…
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I know there has been a thread about this in the past: https://github.com/trinityrnaseq/trinityrnaseq/issues/1231
but there seems to me to be an issue that isn't clearly resolved or explained.
We …
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I have ran trinity based fusion transcript denovo assembly, but I am wondering is there a way to identify the fusion break point within this transcript sequence? In another words, if I have a fusion t…
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Hi,
We have a de novo trinity assembly from RNAseq data and also pacbio isoseq assembly. But we do not have the genome.
Can we combine these two to build a comprehensive transcriptome??
Thank…
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Greetings, I didn't find any official contacts, e.g. mailing, so writing my question here... First, thank you a lot for such user friendly developed software! Appreciate it!
Second... Our junior bi…
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Dear Professor Chen,
First of all, I would like to thank you and your team for developing ERVcaller, which has great assisted my project.
Recently, I am trying to assemble the inserted virus seq…
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I have ran trinity based fusion transcript denovo assembly, but I am wondering is there a way to identify the fusion break point within this transcript sequence? In another words, if I have a fusion t…
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Hi,
I'm using Mash to detect contamination in de-novo genome assemblies, together with other tools that work on the latest release of the RefSeq database. Is it possible to build a sketch file for th…
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hello,
I want to run necklace using de novo assembly fasta files, I am running necklace with the -p option and I have created the configuration file with the path to the de_novo_assembly_files and …