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Hi,
When I do the gRNA design for short sequence where only 2-3 gRNA exist, it worked very well. However, when I increased the input sequence length and there were 19 gRNA, the scores and the numbe…
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Hi,
I am trying CB2 analysis in R to analyze my CRISPR data.
The package is installed properly (I guess) and example data runs fine.
When I am trying my own data, I can load the files correctly. bu…
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### Error: mageck mle; how to solve this problem?
> mageck mle --count-table BatchCorrect.txt \
> --design-matrix designmatrix.txt \
> --norm-method control \
> --control-sgrna ctrl_sgrna_list…
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Hi I am trying to get gene stats from the code but ı get this following error;
```
Error in measure_gene_stats(sgrna_stat) :
It looks like `sgrna_stat` does not contain any result of a statist…
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Hello,
I am trying to use JACKS and running into following issues:
```
python ~/Research/Programs/Jacks/JACKS/jacks/run_JACKS.py Count_Matrix.tab Exp_Summary_JACKS.tab sgRNA_Mapping_File.tab --sgrn…
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I'd like to be able to design CRISPR guide RNAs with Poly. Below is a draft spec for a new CRISPR package:
## Spec
Our guide RNA design package should be lightweight and easily extendable and at…
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Hi! Thanks for developing such a comprehensive tool for sgRNA design. I am wondering if it is possible to add on-target scores for SaCas9 using models like Azimuth 2.0 or something else. It will be ve…
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Hello,
First of all, thank you for your tool it is very usefull. I am trying to use it for a custom genome. I build the necessary BSgenome library and install my genome successfully.
I success…
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### Description of feature
I have some non-target sgRNA in my library.
megeck count privide parameters [`--control-sgrna`](https://sourceforge.net/p/mageck/wiki/QA/#what-does-the-control-sg…