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Issue Documentation is http://handbook.arctosdb.org/how_to/How-to-Use-Issues-in-Arctos.html
**Goal**
More truthfully reflect the nature of environmental samples, soil samples in this case.
**Co…
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I believe this to be a sequencing artifact rather than an issue with DADA2, but perhaps you have advice on how best to mitigate this issue computationally. I did an experiment where I basically challe…
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I did not download the MiSeq data file. Here is the command I followed:
(base) [fahmi@rackham3 Metabarcoding]$ wget http://www.mothur.org/w/images/d/d6/MiSeqSOPData.zip
--2020-02-19 19:04:25-- …
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Hello,
I constructed simple mock communities comprised of short synthetic genes that vary only at a 6 bp region in the middle, combined in various known concentrations (example sequences of one suc…
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SMEA postdoc reached out to me. Asking for rec.s of more specific assay design for _Hematodinium_. Message snippet below:
> We recently did a large eDNA metabarcoding study and found a single sequ…
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As I explain to other researchers the merits of DADA2, I am always at a loss as to what to call the final sequences from the DADA2 results. I usually just say "OTU", because it is easy to understand, …
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I've seen these primers used in several studies, so you might want to consider including them in the 18S primer database. The original publication seems to be:
Vainio EJ, Hantula J (2000) Direct an…
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Session Info
```r
> devtools::session_info()
- Session info ---------------------------------------------------------------------------------------
setting value
…
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Hi,
I am trying to use BURRITO on my PICRUSt2 output. I used SILVA for the affiliation step and FROGS for the whole metabarcoding analysis. When I tried to upload the files to BURRITO I got the fol…
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I'm trying to merge two phyloseq objects (from 16S data produced using the same closed reference pipeline, just different runs) using merge_phyloseq. The problem is that the resulting merged object do…