-
**Describe the bug**
When trying to simulate reads with length greater than 400, I get an IndexError: list index out of range error
**To Reproduce**
Steps to reproduce the behavior:
` python gen…
-
### Description of feature
Would it be possible to update the V1200 scheme to the newest version (v2 currently) that allows better sequencing of Omicron?
The V1200_v2 files can be found here from…
-
I have a two-part question about small batches where processing 5-6 controls is not financially feasible. Context shall follow.
1. Is the recommendation for 5-6 controls a relatively fixed value or…
-
I would like to use LACA for shorter amplicons (600 bp) from ONT sequencing.
Can you provide the following options in the laca init so that the config file can work with alternative sequence input…
-
Hi,
My sequencing data is amplicon sequencing that some regions got excessive coverage. I wonder if I can set a maxi coverage in Pisces.
Thank you!
-
Currently we generate the mapping files for an amplicon run with the `amplicon-pooling.ipynb` notebook, however this should be changed to work the same was as with metagenomics runs. Namely using the …
-
Hi there!
I utilized EMU to classify 16S and 18S rRNA amplicons sequenced using nanopore sequencing with the SQK-NBD114.96 kit. However, I noticed that some of the taxonomic assignments made by EMU …
-
I am using a custom amplicon panel for wastewater sequencing and would like to know the process of adding this to the primer sets folder.
E.g. The naming conventions needed and how to go about gene…
-
Hi there,
I browsed your interesting [paper](https://doi.org/10.1093/nar/gkad158) (that isnt mentioned in the README) and was intrigued by the speed improvement while promising a similar performanc…
-
Dear Developers,
I'm trying this pipeline. However, when I typed
python ./scripts/Master.py -h
, it turned out:
Traceback (most recent call last):
File "./scripts/Master.p…