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@sbooeshaghi, I went through your [notebook](https://github.com/pachterlab/MBLGLMBHGP_2021/blob/master/notebooks/retina_velocity.ipynb) on the mouse retinal cell analysis. During the normalization of …
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Hi,
I am using the 0.48.0 version of kallisto, as well as bustools (0.41.0) to demultiplex and obtain gene count tables for my BD Rhapsody WTA data. This is my initial kallisto bus script:
**kallis…
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Hello,
First of all, I love bustools.
With that said I ended up with bustools 0.41.0 on my laptop and it is lovely. I have no idea how it got there, all of my other machines have an older versi…
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Hi kite authors,
Thanks for developing a useful tool.
Do you all have a preferred citation for `kite`?
I've already cited `kallisto` and the `bustools` papers, but for `kite` I'm using the Gith…
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Dear BUStools team,
Could you please provide links to intron indexes for both mouse and human?
In your [tutorial](https://github.com/BUStools/getting_started/blob/master/kallisto_bus_mouse_nucle…
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Hey,
First of all thanks for developing and implementing Kallisto. It is an amazing tool!
I just want to confirm a couple of things regarding the assignment of reads mapping to two transcripts f…
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I'm trying to perform RNA velocity with kallisto, bustools and their wrapper kb-python following the instructions in [this R Notebook](https://bustools.github.io/BUS_notebooks_R/velocity.html). But I'…
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Hi,
I have an experiment wih a whitelist composed of 35 barcodes (60bp long, non standard technology in development) and when I try to run bustools correct I immediately obtain :
(/bioinfo/local…
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Hello,
I am currently following the `kallisto | bustools` tutorial on processing nuclear RNA-seq, which runs `bustools count` on spliced and unspliced transcripts. I know that you can specify `--g…
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[update]: oops, I just realized you can use `kallisto quant-tcc` to do this
I know there are workarounds for this but it would be nice to have a `--transcript-counts` option for `bustools count` th…