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According to https://github.com/COMBINE-lab/salmon/issues/434#issuecomment-540533728 the current behaviour of the Alevin wrapper in which each fastq file provided in a collection is analysed separatel…
pcm32 updated
5 years ago
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Hi,
I would like to test your tool. How can create the two input files. I have the results from a cellranger run, i.e. bam, fastq, and count matrix.
Thx
Bernd
baj12 updated
3 years ago
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Before this change https://github.com/samtools/htslib/pull/1854 U was changed to N when read by samtools
Now it will be changed to T
However, I think it would be "better" if we could preserve U …
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Hi,
I have Nanopore sequencing data for the 16s gene of microbioal communities. Can dada2 be used for this type of sequencing data, and if not, is it in development? Thanks!
Best wishes,
Carla …
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Hi @lh3 @tanlongzhi
I'm a little confused that the strand for R2 was reversed here. Did I miss anything?
`// read_num for PE sequencing: 1 for R1, 2 for R2
var read_num = flag >> 6 & 0x3;
…
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Dear all,
We have been going through the SAM specification with a fine comb, and
found a few issues.
The main issue is that there seems to be a confusion about ‘read’ and
‘segment’ at various plac…
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**Description**
As a data wrangler wrangling a project with 10X 5' V(d)J data I need to determine the correct way of defining the experiment in our spreadsheet and determine whether any a…
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This issue contains monthly updates to the ranked list of PubMed papers for curation. The full list can be found [here](https://github.com/tanayshah2/bioregistry/blob/5560dcf284f2c0309287e0a5a551e435e…
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Hello,
Question about 'RNA sequencing assay' (OBI:0001177) and 'RNA-seq assay' (OBI:0001271). In OBI, 'RNA-seq assay' is a subclass of 'RNA sequencing assay'. Are there examples of assays that fal…
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Preferred Term: m6A-MTase sequencing assay
Synonyms: Fiber-seq
Definition: A DNA methylation profiling assay in which chromatin stencils are created by treating nuclei with m6A methyltransferase (MT…