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The CRISPOR website provides warnings of potential problems with gRNA sequences, such as presence of TTTT that terminates transcription from U6 promoters, but I do not see these warnings in the output…
genya updated
5 years ago
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Hi Aaron,
I am running into a error while testing FlashFry to look for gRNA in my dataset. I have 12 sequences(100bp/each), use hg38 assembly. I was able to get the "discover" function to work (out…
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The production interaction in this file is not being rendered properly. The RNA and DNA are rendered on top of each other.
[RGR_13_grna_rna_production.zip](https://github.com/VisBOL/visbol-js/file…
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The degradation interaction for RNA is not centered with the RNA glyph.
[RGR_11_grna_rna_degradation.zip](https://github.com/VisBOL/visbol-js/files/2532614/RGR_11_grna_rna_degradation.zip)
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Linking experimental data to samples and genetic designs is becoming critical to many synthetic biology projects.
We thus propose to add two new classes, Experiment and ExperimentalData, to enable …
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potentially related to datacite/bolognese#36
[Front conversations](https://app.frontapp.com/open/top_facz)
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The SO term is non-coding RNA and not gRNA, so the abbreviation should be ncRNA.
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I'm wondering how we're supposed to encode epigenetic information for passing into the "off-target" version of the pipeline. The model wants both the sgRNA and the target as 8-channel vectors that inc…
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I get this exception when setting an id:
java.lang.IllegalArgumentException: org.sbml.jsbml.IdentifierException: Cannot set duplicate meta identifier RGR_12_grna_rna_dCAS9_Mxi1_protein_complex_form…
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We propose to introduce a new class, tentatively called **Implementation**, to represent different replicates of physical design realizations. In practice, `Implementation` will identify cell clones o…
graik updated
5 years ago